vaxarray analysis software v2.2 (InDevR Inc)
Structured Review

Vaxarray Analysis Software V2.2, supplied by InDevR Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vaxarray analysis software v2.2/product/InDevR Inc
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "30-Minute Highly Multiplexed VaxArray Immunoassay for Pneumococcal Vaccine Antigen Characterization"
Article Title: 30-Minute Highly Multiplexed VaxArray Immunoassay for Pneumococcal Vaccine Antigen Characterization
Journal: Vaccines
doi: 10.3390/vaccines10111964
Figure Legend Snippet: Design of VaxArray pneumococcal assay. ( a ) Schematic representation of the VaxArray pneumococcal assay kit microarray slide showing 16 replicate microarrays, A1-H2 represents the location of each microarray; ( b ) individual microarray layout showing 3 replicate spots for each serotype, with fiducial markers in grey at top and bottom; and ( c ) general assay detection scheme showing serotype-specific capture with primary detection and fluorescent secondary detection label. The primary label can either target polysaccharide or CRM197 carrier protein. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).
Techniques Used: Microarray
Figure Legend Snippet: Representative fluorescence microarray images demonstrating reactivity and specificity of the VaxArray pneumococcal assay after the incubation of 2 µg/mL monovalent Pfizer native PS serotypes 1, 2, 3, 4, 5, 6A, 6B, 7F, 8, 9N, 9V, 10A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F, and 33F (ATCC, deposited by Pfizer); a no antigen blank; and a 23-valent mixture containing all serotypes mentioned above.
Techniques Used: Fluorescence, Microarray, Incubation
Figure Legend Snippet: Native PS reactivity. Signal-to-blank ratios (S/Bl) on the VaxArray 23-valent pneumococcal assay for Pfizer native PS materials (ATCC, deposited by Pfizer) at 2 µg/mL; columns represent each serotype-specific capture antibody, and rows represent each serotype of native PS tested. Text in bold green shows S/Bl ≥ 3 generated for the matched PS and capture antibody. Text in bold black shows S/Bl ≥ 3 generated on off-target capture antibody indicating cross-reactivity.
Techniques Used: Generated, Microarray
Figure Legend Snippet: Signal correlation between anthrone and VaxArray assays. ( a ) Signal response correlation for S6A native PS from EuBiologics (EuB). The same serial dilution evaluated by the anthrone assay was diluted 100- to 200-fold to perform VaxArray assay due to VaxArray assay sensitivity. Error bars indicate ± 1 standard deviation (n = 3). ( b ) Precision (%RSD) for anthrone and VaxArray assays and associated R2 value for signal correlation of each sample (n = 3 for each concentration of each method).
Techniques Used: Serial Dilution, Standard Deviation, Concentration Assay
